A gold bar, eight gold chains and 11 ornate gold pieces were among the artifacts divers from Blue Water Ventures said they found earlier this week.
Duncan Mathewson, a marine archaeologist and partner in Blue Water Ventures, said the artifacts are from the Spanish galleon Santa Margarita, which sank during a storm in 1622.
The treasures were found in nearly 18 feet of water about 40 miles west of Key West, Mathewson said.
"We're following the road to the treasure," Mathewson said. "We're on the trail, right smack where we need to be."
An initial cache of treasure and artifacts from the galleon was discovered in 1980 by the late treasure-hunting pioneer Mel Fisher.
Blue Water Ventures has been searching for the remainder of the wreck for two years under a joint-venture agreement with Mel Fisher's Treasures, now headed by Fisher's son, Kim Fisher.
The artifacts and treasure were taken to Fisher's Key West headquarters Thursday for conservation. Mathewson estimates more than $150 million to $200 million worth of artifacts and treasure from the Santa Margarita remains to be recovered.